- Original Article
- Construction and Application of Painting probe for
Chromosome band 5q14-q15, 6q23, 16p11.2 and 22q12
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Soo-Yeun Park, So Young Park, Joon-Il Kil, Yong-Hyuck Chun, Sun-Hwa Park
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Clin Exp Pediatr. 2000;43(2):179-187. Published online February 15, 2000
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Purpose : Chromosome microdissection has become a very powerful approach to generate chromosome band-specific library and painting probes for physical mapping or cytogenetic analysis. We have constructed here band-specific painting probes for human chromosomes by microdissection and polymerase chain reaction(PCR).
Methods : We pretreated the microdissected fragments with Topoisomerase I(Topo I) which catalyzes the relaxation of supercoiled DNA, and performed... |
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